ABSTRACT
OBJECTIVE:To establish the quality standard for Embelia laeta. METHODS:TLC was adopted for the qualitative identification. HPLC was adopted for the content determination of embelin:the column was Waters C18 with mobile phase consisted of methanol-5%methanoic acid(90∶10,V/V)at a flow rate of 0.9 ml/min,the detection wavelength was 288 nm,the column tem-perature was 40 ℃,nd the injection volume was 10 μl. RESULTS:The TLC of E. laeta showed clear spots and good separation. The linear range of embelin was 4.012-40.12 μg/ml(r=0.999 4);RSDs of precision,stability and reproducibility tests were lower than 2%;recovery was 96.54%-99.57%(RSD=1.20%,n=6). CONCLUSIONS:The established standard can be used for the quality control of E. laeta.
ABSTRACT
OBJECTIVE: To study the chemical constituents of the fruits of Embelia laeta. METHODS: The compounds were isolated and purified by MCI, medium pressure TLC on silica gel column, ODS column chromatography, semi-preparative HPLC, and so on. The structures were elucidated on the basis of various modern spectroscopic techniques using physical, chemical properties and spectral data. RESULTS: Fifteen compounds were isolated and identified as nantenine(1), oxonantenine(2), physcion(3), syringic acid(4), vanillic acid(5), stigmast-4-ene-3,6-dione(6), (+)-lyoniresinol(7), 75,85-threo-4,7,9,9'-tetrahydroxy-3,3'-dimethoxy-8-O-4'-neolignan(8), 1,3-dihydroxylpropyl-(9Z,12Z)-octadeca-9,12-dienate(9),(22E)-5a,8a-epidioxyergosta-6,22-dien-3b-ol(10), dihydroxyisoechinulin A(ll), hydroxybenzoic acid(12), stigmasterol(13), sitosterol(14), and daucosterol(15). CONCLUSION: Compounds 1,2, and 5-13 are isolated from the genus Embelia for the first time, and compounds 1-15 are isolated from this plant for the first time.
ABSTRACT
The plants in Embelia Burm. f. such as E. parviflora and E. laeta are commonly used medicines and their therapeutical effects have been confirmed in the Zhuang medicine. At present, there are researches showing that plants in Embelia Burm. f. have similar chemical composition and pharmacological effect, so the plants have certain value of development and utilization. In this article the pharmacognosy, chemical composition, quality analysis, and pharmacological effect of plants in Embelia Burm. f., as well as the development and research of the fruit were summarized.